Protein Modification Reagents
Thermo Scientific™ 8M Guanidine-HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ MMTS (methyl methanethiosulfonate)
Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ Iodoacetic Acid
Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.
Thermo Scientific™ Pierce™ Cell Surface Biotinylation and Isolation Kit
The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific™ Pierce™ Cysteine-HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).
Thermo Scientific™ Pierce™ Alkylating Reagents
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.