Crosslinking, Labeling and Protein Modification
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Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | Sulfo-SMCC |
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| PEGylated | No |
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin
Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
| Cell Permeability | Cell-Impermeant |
|---|---|
| Content And Storage | Store at -20°C. |
| Product Type | NHS-PEG4-Biotin |
| Label or Dye | Biotin |
| Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
| Chemical Reactivity | Amine |
| Product Line | EZ-Link™ |
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store at -20°C protected from light and moisture. |
| Cleavable | By Thiols |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 492.4 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Nonselective |
| Cell Permeability | No |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Spacer Arm Length | 18.2 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Optimize applications where product integrity and risk minimization are paramount with this high-quality formulation of carbodiimide crosslinker.
| Content And Storage | Upon receipt store desiccated at -20°C. |
|---|---|
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| Product Line | Pierce™ |
| PEGylated | No |
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
| Cleavable | No |
|---|---|
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 326.26 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 7.7 Å |
| PEGylated | No |
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
| Label Type | Biotin & Analogs |
|---|
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
| Content And Storage | Store at -20°C. |
|---|---|
| Product Type | Protease |
| Form | Kit |
| Product Line | Pierce™ |
Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format
Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.
| Content And Storage | Upon receipt store desiccated at 4°C. |
|---|---|
| Description | DSP |
| Cleavable | By Thiols |
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 404.42 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 12.0 Å |
| PEGylated | No |