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Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Brand: Thermo Scientific™ 22582
Additional Details : Weight : 1.81440kg
Description
A solution of this compound produces a measurable yellow-colored product when it reacts with sulfhydryls. Consequently, Ellman's Reagent is very useful as a sulfhydryl assay reagent because of its specificity for –SH groups at neutral pH, high molar extinction coefficient and short reaction time. Sulfhydryl groups may be estimated in a sample by comparison to a standard curve composed of known concentrations of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups may be quantitated by reference to the extinction coefficient of TNB.Highlights:
Sulfhydryl detection– reacts quantitatively with free (reduced) sulfhydryl groups
Colorimetric–yields colored product to enable spectrophotemetric measurement in tubes or microplates
Proven assay method– well-characterized chemistry enables quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
Specifications
| Visible absorption (412nm) | |
| 5,5's-Dithio-bis-(2-nitrobenzoic acid); powder (at least 99% pure) | |
| See MSDS | |
| 5g | |
| 4°C | |
| 4°C |
| Pierce DTNB (Ellman's Reagent) | |
| Protein or peptide sulfhydryl assays | |
| 5,5's-Dithio-bis-(2-nitrobenzoic acid) | |
| Pure | |
| Preparing 1.25L of typical 1X assay solution |