Protein Labeling Reagents
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Cytiva CypHer5E NHS Esters
A red-excitable, pH-sensitive cyanine dye derivative, reporting receptors movement from the cell surface into acidic endosomes upon agonist stimulation.
| Product Type | CypHer5E NHS Ester |
|---|---|
| Formulation | 100% CypHer 5E |
| Color | Blue |
| Solubility | Easily soluble in methanol; Very slightly soluble in cold water and hot water. |
| Emission | 663 |
| For Use With (Application) | This reagent has been shown to be suitable for live cell receptor internalization studies using the IN Cell Analyzer 3000, IN Cell Analyzer 1000, and confocal microscopes. 0.5 mL of stock reagent will be sufficient for up to 500 wells. |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Hydrazide)
For the labeling of free carbonyl groups of glycoproteins and carbohydrates
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
|---|---|
| pH Range | 3 to 10 |
| Solubility | Soluble in cold water and hot water |
| For Use With (Application) | For labelling via carbonyl groups. For use in steady state fluorescence, FP and FRET applications |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive Maleimide)
For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides
| Kit Contents | Foil packs, each containing 1, 5 or 25mg dried dye |
|---|---|
| pH Range | 3 to 10 |
Cytiva Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye 5-packs
More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye packs are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
| Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
|---|---|
| Kit Contents | Five vials of dye |
| Solubility | Soluble in cold water and hot water |
| Sufficient For | Labeling 1mg Protein |
| Quantity | 5 vials each sufficient to label 1 mg protein |
Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of premeasured dye |
|---|---|
| Format | Powdered |
| pH Range | 3 to 10 |
| Product Type | Cy5.5 NHS Ester |
| Color | Blue |
| Label or Dye | Cy5.5 |
Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester)
Use for highly accurate labeling of amine groups. Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester) contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs, each containing 1, 5, 10, 25 or 50mg dried dye |
|---|---|
| pH Range | 3 to 10 |
Goat anti-Mouse IgG Fluor-Linked Secondary Antibody Conjugate, Cytiva
Affinity purified Cy2-linked secondary antibody conjugate
Cytiva Amersham™ CyDye™ Antibody Labeling Kits
Convenient way to tag antibodies with CyDye fluorescent dyes
| Content And Storage | 2°C to 8°C, protected from light |
|---|---|
| pH Range | 7 to 7.3 |
| Quantity | Each |
Cytiva Cy™3B Mono-reactive Maleimide Fluorescent Dyes
Combines exceptional photostability with bright and intense signals. Cytiva Cy3B Maleimides Mono-Reactive CyDye is a more selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions.
Cytiva Amersham™ CyDye™ Reactive Dye 5-Packs
Use for the most accurate labeling. Cytiva Amersham™ CyDye™ Reactive Dye 5-Packs contain derivatized CyDye that carries only one reactive group on each dye molecule for accurate labeling of amine groups.
| Kit Contents | Five foil packs each containing dried dye to label 1mg of Protein |
|---|---|
| Sufficient For | Labeling 1mg Protein or 30nM Amino groups |
| Quantity | Each |
Cytiva Cy3 NHS Ester Mono-Reactive CyDye
Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
| Kit Contents | Foil packs of pre-measured dried dye |
|---|---|
| Format | Dried |
| pH Range | 3 to 10 |
| Product Type | Cy3 NHS Ester |
| Color | Red |
| Label or Dye | Cy3 |
| Kit Contents | Foil packs, each containing 1, 5, 10, 25 or 50mg dried dye |
|---|---|
| Product Type | Cy3.5 NHS Ester |
| Formulation | 100% Cy3.5 (NHS ester) |
| Color | Red |
| Label or Dye | Cy3.5 |