Miscellaneous Molecular Biology Reagents
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Thermo Scientific™ EDTA
Chelate alkaline earth metals. Thermo Scientific™ Pierce EDTA is highly purified for use in a variety of laboratory methods.
Thermo Scientific™ IPTG, dioxane-free
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Thermo Scientific™ MassRuler DNA Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.
Thermo Scientific™ 5-Fluoroorotic Acid
Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ Yeast DNA Extraction Kit
Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.
Polysorbate 20
CAS: 9005-64-5 Molecular Formula: C26H50O10 Synonym: Polyoxyethylene(20)sorbitan monolaurate
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Thermo Scientific™ DNA Loading Dye & SDS Solution (6X)
Perform agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins with this DNA loading dye containing SDS.
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ Agarose I (Molecular Biology Grade)
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.