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Thermo Scientific™ X-Gal Solution, ready-to-use
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Description
Formula: C14H15BrClNO6 Molecular weight: 408.6
X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of the lacZ gene with IPTG leads to the hydrolysis of X-Gal and to the development of blue colonies (see Supporting data).
X-Gal Solution, ready-to-use, is stable, 0.22 μm membrane filtered solution formulated for direct use in conjunction with IPTG for blue/white colony screening.
Recommended for:
Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (1); Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells; Detection of beta-galactosidase activity in immunological and histochemical procedures.
Note:
Preparation of a 20mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF-containing solution to plastic Petri dishes should be avoided. DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.
Specifications
Specifications
| Detection Method | Colorimetric |
| Product Type | X-Gal Solution |
| Quantity | 10 mL |
| Substrate Properties | Chemical Substrate |
| Substrate Type | Beta-Gal Substrate, X-Gal |
Safety and Handling
- X-Gal Solution, ready-to-use
- Warning
- Flammable liquid Category 3
- Skin sensitiser Category 1
- H226-Flammable liquid and vapour.
- H317-May cause an allergic skin reaction.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P370+P378-In case of fire: Use dry sand, dry chemical, or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol, hydroquinone, DMSO
For Research Use Only. Not for use in diagnostic procedures.
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