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TaqMan™ SCID/SMA Plus Assay

Product Code. 31722835 Shop All Applied Biosystems Products
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Product Code. 31722835 Supplier ABI Applied Biosystems Supplier No. A48566

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Description

A multiplex real-time PCR assay that detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control.

The Applied Biosystems TaqMan SCID/SMA Plus Assay is a multiplex real-time PCR assay that detects the SMN1 gene, TREC, and KREC, as well as the RNase P gene as an internal genomic control. For an assay that detects only SMN1 and TREC, please see the TaqMAN SCID/SMA Assay (Cat. No. A48566).

Features of the TaqMan SCID/SMA Plus Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automation

Complete solution from DBS to result
The TaqMan SCID/SMA Plus Assay detects SMN1, TREC, and KREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus an SMN2 blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from 96- to 384-well plates is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • DNA Extract All Reagents Kit
• TaqPath ProAmp Multiplex Master Mix
• TaqMan SCID/SMA Plus Assay

Upon receipt, store at -20°C. After opening, store at 4°C.
Detection Method Primer-probe
For Use With (Equipment) QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Product Line TaqMan
Quantity 1,000 reactions
Shipping Condition Dry Ice
No. of Reactions 1000 Reactions
Sample Type DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.

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