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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (1,222)
Thermal Cycler Accessories (213)
Thermal Cyclers (205)
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Keyword Search:
1 – 15 of 997 results
1
Promotions Available

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
2
Promotions Available

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2X
For Use With (Application) Standard PCR,High-fidelity PCR
3
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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
4
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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE
Shipping Condition Wet Ice
InhibitorToleranceSimple Medium
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
5
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Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
6
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Applied Biosystems™ QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.1 mL

Plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1mL RT-PCR systems.

EDGE
7
Promotions Available

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

EDGE
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Concentration 5 U/μL
8
Promotions Available

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
9
Promotions Available

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
10
Promotions Available

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

EDGE
Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
11
Promotions Available

Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

EDGE
12
Promotions Available

Applied Biosystems™ Protein Thermal Shift™ Dye Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

EDGE
13
Promotions Available

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
14
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

EDGE
GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
15
Promotions Available

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

EDGE
Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C in the dark.
InhibitorToleranceSimple Very High
For Use With (Application) Genotyping
PCR Method qPCR
Multiplex Capability 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line TaqPath