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IBA Lifesciences StrepMAB-Immo Conjugate
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Product Code. 16421475 Shop All IBA Lifesciences Products
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Label or Dye:
DY-488
DY-549
DY-649
Packungsgröße:
50µg
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Artikelnummer. Label or Dye unitSize
16421475 DY-488 50µg
16451475 DY-549 50µg
16481475 DY-649 50µg
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Artikelnummer. 16421475 Lieferant IBA Lifesciences Lieferanten-Nr. 21564050

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Beschreibung

StrepMAB-Immo conjugates are monoclonal antibodies with different fluorescent conjugates to detect Strep-tag™II and Twin-Strep-tag™ fusion proteins

StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry.

The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tag™II providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein).

Proteins containing Strep-tag™II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far, the Twin-Strep-tag™ always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649).

The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm

Please note: For detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin™ is recommended.

Spezifikation

Capacity (Metric) 0.10 mL
Concentration 0.5 mg/mL
Kit Contents Solution in PBS; 0.01 % NaN3; 1 mg/ mL BSA
Content And Storage Undiluted: 2°C to 8°C, protect from light; Diluted: -25°C to -15°C
Quantity 50 μg
For Use With (Application) ELISA, FACS, immunofluorescence
Target Strep-tag™II and Twin-Strep-tag™
Type Conjugate
Label or Dye DY-488

For Research Use Only.

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