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  5. IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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Code produit. 15693878 Tous les produits IBA Lifesciences Produits
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Vector:
pDSG-IBA102
pDSG-IBA103
pDSG-IBA104
pDSG-IBA105
pDSG-IBA43
pDSG-IBA45
pDSG-IBAwt1
pDSG-IBAwt2
Protein Tag:
His Tag (6x), Strep-tag II
Twin-Strep-tag
Untagged
Conditionnement:
5µg
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Code produit. 15693878

Marque: IBA Lifesciences 55211001

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Description

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Spécification

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species Mammalian
Protein Tag His Tag (6x), Strep-tag II
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer pENTRy-IBA51 Reverse Sequencing
Quantity 5 μg
Vector pDSG-IBA43
For Use With (Application) Vector for recombinant expression in mammalian cells
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