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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL)
A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.
Brand: Thermo Scientific™ EP0451
116.55 EUR valid until 2023-12-01
Use promo code "21275" to get your promotional price.
Additional Details : Weight : 0.97980kg
Description
RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Highlights
- High yields of full-length first strand cDNA up to 13 kb
- Optimum activity at 42 to 45°C
- Active up to 55°C
- Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)
Specifications
• RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|
| 60 min. | |
| 42°C to 45°C | |
| RevertAid H Minus | |
| Dry Ice | |
| First-Strand cDNA | |
| Reverse Transcription | |
| RNA |
| Stand-alone Enzyme | |
| Reverse Transcription | |
| 10,000 Units | |
| Reduced | |
| 200 U/μL | |
| Separate components | |
| Up to 13 kb |