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R&DSystems™ Recombinant Human R-Spondin 3 Fc Chimera Protein, CF

Product Code. 30023140
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Quantity:
100 μg
20 μg
50 μg
500 μg
Unit Size:
100µg
20µg
500µg
50µg
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Product Code. 30023140

Brand: R&DSystems™ 11506RS020

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Description

Recombinant Protein

Measured by its ability to activate TCF reporter activity in HEK293 human embryonic kidney cells in the presence of Recombinant Human Wnt-3a (Catalog # 5036-WN ). The ED50 for this effect is 0.500-10.0 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9BXY4.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 84870
Molecular Weight (g/mol) M.W.-observed: 53-69 kDa, under reducing conditions., M.W.-theroretical: 46 kDa
Quantity 20 μg
Source Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 3 protein Human RSPO3 (Met33-Gly209) (N-terminus) Accession # Q9BXY4.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias Cristin 1, CRISTIN1, FLJ14440, hPWTSR, hRspo3, Protein with TSP type-1 repeat, PWTSR, Roof plate-specific spondin-3, RSPO3, RSpondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, Thrombospondin type-1 domain-containing protein 2, thrombospondin, type I, domain containing 2, THSD2
Conjugate Unconjugated
Reconstitution Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein R-Spondin 3
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