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R&D Systems™ Recombinant Human Notum Protein

Product Code. 18747817
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Quantity:
50 μg
Unit Size:
50µg
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Product Code. 18747817

Brand: R&D Systems™ 9118NM050

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This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Notum Protein is derived from CHO. The Recombinant Human Notum Protein has been validated for the following applications: Bioactivity.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q6P988
For Use With (Application) Bioactivity
Gene ID (Entrez) 147111
Molecular Weight (g/mol) Observed M.W.: 38-45kDa, reducing conditions, Theoretical M.W.: 43kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line,CHO-derived human Notum protein Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog
Common Name NOTUM
Biological Activity Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.1-0.6μg/mL in the presence of 0.5μg/mL of Recombinant Human Wnt-3a.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived Ser81-Thr451 (Cys330Ser) with a C-terminal 6-His tag
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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