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R&D Systems™ Recombinant Human IL-4 Protein, Animal-Free

Product Code. 18795865
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Quantity:
1 mg
50 μg
Unit Size:
1mg
50µg
This item is not returnable. View return policy

Product Code. 18795865

Brand: R&D Systems™ BT004AFL01M

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This item is not returnable. View return policy

Intended for preclinical researchers who may transition to GMP IL-4 for their clinical work

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.0400-0.320 ng/mL. The specific activity of Animal-FreeTM Recombinant Human IL-4 is >1.0x107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05112.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 3565
Molecular Weight (g/mol) M.W. (Observed): 13 kDa, under reducing conditions., M.W. (Theoretical): 15.1 kDa
Quantity 1 mg
Source E. coli-derived human IL-4 protein His25-Ser153, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 500μg/mL in PBS.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein IL-4
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