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R&D Systems™ Recombinant Human IL-2 GMP Protein, CF

Product Code. 30022049
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50 μg
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50µg
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30022049 50 μg 50µg
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Product Code. 30022049 Supplier R&D Systems™ Supplier No. BT002GMP050
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    413.70€ / 50µg
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Estimated Shipment: 14-09-2026
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Description

Animal-Free. Contains the Cysteine to Serine substitution found in Proleukin™ (aldesleukin)

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.

The ED50 for this effect is 0.0300-0.250 ng/mL.

The specific activity of recombinant human IL-2 is >5.00 x 106 IU/mg, which is calibrated against an internal reference standard value assigned against the human IL-2 WHO International Standard (NIBSC code: 86/500).

TRUSTED_SUSTAINABILITY

Specifications

Accession Number P60568.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with Trehalose.
Gene ID (Entrez) 3558
Molecular Weight (g/mol) Observed: 13 kDa, under reducing conditions., Observed: 15.5 kDa
Quantity 50 μg
Source E. coli-derived human IL-2 protein Ala21 - Thr153 (Cys145Ser), with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
Regulatory Status GMP
Endotoxin Concentration <0.01 EU per 1 μg of the protein by the LAL method.
Gene Alias aldesleukin, IL2, IL-2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, Proleukin, T cell growth factor, T-cell growth factor, TCGF
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 500 μg/mL in sterile deionized water.
Purity or Quality Grade >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 15521 ± 5 Da, and a second 15390 ± 5 Da product may be present
Protein IL-2
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