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R&D Systems™ Recombinant Human FGF basic/FGF2 Protein, CF

Product Code. 18317497
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Quantity:
1 mg
100 μg
20 μg
250 μg
50 μg
Unit Size:
100µg
1mg
20µg
250µg
50µg
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Product Code. 18317497

Brand: R&D Systems™ BTFGFB050

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This item is not returnable. View return policy

Description

Carrier-Free Recombinant Protein

Measured by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120; Watson, J.B. et al. (1994) J. Clin. Invest. 94:261. The ED50 for this effect is 0.100-1.00 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_001997.5
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Gene ID (Entrez) 2247
Molecular Weight (g/mol) M.W. (Observed): 18 kDa, under reducing conditions., M.W. (Theoretical): 16 kDa
Quantity 50 μg
Source E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.01 EU per 1 μg of the protein by the LAL method.
Gene Alias Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
Conjugate Unconjugated
Reconstitution Reconstitute the 20 μg size at 100 μg/mL in sterile deionized water. Reconstitute all other sizes at 500 μg/mL in sterile deionized water.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein FGF basic/FGF2/bFGF
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