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R&D Systems™ Recombinant Human BMP-4 Protein
Description
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Specifications
Specifications
| Accession Number | Q53XC5 |
| Characterization | Bioactivity |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA. |
| Gene ID (Entrez) | 652 |
| Molecular Weight (g/mol) | M.W. (Observed): 19-24 kDa, reducing conditions 35-41 kDa, non-reducing conditions |
| Quantity | 500 μg |
| Source | Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408 |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. |
| Endotoxin Concentration | <0.01EU per 1μg of the protein by the LAL method. |
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