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R&D Systems™ Recombinant Human BMP-4 Protein

Product Code. 18156870
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Quantity:
1 mg
10 μg
50 μg
500 μg
Formulation:
Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
Unit Size:
10µg
1mg
500µg
50µg
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Product Code. 18156870

Brand: R&D Systems™ 314BP01M

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This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q53XC5
Characterization Bioactivity
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Gene ID (Entrez) 652
Molecular Weight (g/mol) M.W. (Observed): 19-24 kDa, reducing conditions 35-41 kDa, non-reducing conditions
Name BMP-4
Quantity 1 mg
Source Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <0.01 EU / 1 ug of the protein by the LAL method.
Gene Alias BMP2B, BMP-2 B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2 B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME
Gene Symbol BMP4
Product Type Recombinant Protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Reconstitution Reconstitute at 50 to 200 ug/mL in sterile 4 mM HCl containing (at least 0.1% human or bovine serum albumin.)
Purity or Quality Grade 95%, by SDS-PAGE with silver staining.
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