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R&D Systems™ Recombinant Human Activin AB Protein, CF
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Quantity:
10 μg
25 μg
Unit Size:
10µg
25µg
Description
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.
Specifications
Specifications
| Accession Number | NP_002183 ( beta A) & NP_002184 ( beta B) |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose. |
| Molecular Weight (g/mol) | M.W.-observed: 14 kDa, reducing conditions, M.W.-theroretical: 12.8 kDa ( beta A subunit) and 13 kDa ( beta B subunit) |
| Quantity | 10 μg |
| Source | Chinese Hamster Ovary cell line, CHO-derived human Activin AB proteinHuman Activin beta A (Gly311 - Ser426) (N-terminus) Accession # NP_002183.1 Human Activin beta B (Gly293 - Ala407) (N-terminus) Accession # NP_002184.2 (C-terminus) |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.10 EU per 1 μg of the protein by the LAL method. |
| Conjugate | Unconjugated |
| Reconstitution | Reconstitute at 100 μg/mL in 4 mM HCl. |
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