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R&D Systems™ Recombinant Cynomolgus Monkey TNF-alpha Protein, CF

Product Code. 18749936
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Quantity:
50 μg
Unit Size:
50µg
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Product Code. Quantity unitSize
18749936 50 μg 50µg
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Product Code. 18749936 Supplier R&D Systems™ Supplier No. 10765TA050

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Description

Recombinant Protein

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.40-4.00 ng/mL.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_001272206.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 7124
Molecular Weight (g/mol) MolecularWeight-observed: 15-17 kDa, under reducing conditions, MolecularWeight-theroretical: 17 kDa
Quantity 50 μg
Source E. coli-derived cynomolgus monkey TNF-alpha protein Val77-Leu233
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein TNF-alpha
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