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R&D Systems™ Recombinant A. tubingensis PNGase Protein

Product Code. 18768609
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20 μg
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20µg
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18768609 100 μg 100µg
18710789 20 μg 20µg
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Product Code. 18768609 Supplier R&D Systems™ Supplier No. 9586GH100

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Description

rFm PNGase F is also available!

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. 50% ribonuclease B (6 μg) is deglycosylated by 500 ng of Recombinant A.tubingensis PNGase within 120 minutes, as measured under the described conditions.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number OJI88926.1
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Molecular Weight (g/mol) M.W. (Observed): 72-85 kDa, reducing conditions; M.W. (theoretical): 60 kDa
Quantity 100 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived a. tubingensis PNGase At protein Leu22-Ser557 (F78I, E352G, P381S, S406Q, T412S, S414F, S451T and S555T), with an N-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Species A. tubingensis
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PNGase At
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