Learn More
Thermo Scientific™ RapidFinder™ Vegan ID Kit
Description
Simply and reliably detect the presence of animal DNA in food samples by real-time PCR using the RapidFinder™ Vegan ID Kit. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder Vegan ID Kit detects animal DNA in both raw and processed foods and provides a sensitivity down to 0.01% of animal DNA to support vegan label claims. The included positive control, which represents a 0.1% v/v of vertebrate animal DNA, can be used as a reference to set the threshold of detection of the assay. Invertebrate animal species such as seafood (crustaceans and molluscs) and terrestrial insects such as worms, crickets, and grasshoppers are not detected by the assay.
The RapidFinder™ Vegan ID Kit uses real-time PCR to target a mitochondrial DNA region which is conserved in vertebrate animal species, providing highly sensitive detection of vertebrate animal species. The kit helps food laboratories verify the vegan status of food and feed products. Additionally, identifying meat species in food samples is a crucial step for verifying the origin and traceability of raw materials, as well as for ensuring quality control in handling and cleaning processes on production lines.
- Simple: Real-time PCR based assay, no electrophoresis needed
- Flexible: Vertebrate animal DNA detected in any food or feed sample, both raw and processed
- Reliable: Internal Positive Control (IPC) provides process-control check for PCR inhibitors
- Fast: From sample to result in the same day
How the RapidFinder™ Vegan ID Kit works
Animal DNA detection is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects one mitochondrial DNA sequence common to all animal species. Also included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.
By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is represented as 0.1% vertebrate animal DNA, and by comparing sample results to the control results, a threshold of detection is provided.
Sample preparation
DNA sample preparation for the RapidFinder™ Vegan ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed foods, as well as composite food samples.
Part of a suite of solutions for meat species detection
The RapidFinder™ Vegan ID Kit is just one of our many kits available for the sensitive detection of various animal species in food and feed samples. Other RapidFinder™ animal species identification kits available include those for the detection of beef, goat, rabbit, water buffalo, duck, pork, horse, sheep, ruminant, chicken, turkey, and poultry.
The RapidFinder™ Animal Species Identification kits are made available through a partnership with Health in Code, S.L.
Specifications
Specifications
| Content And Storage | Contains 1 tube each of: Vegan Master Mix (store at -20°C), General Master Mix (store at -20°C upon receipt; 2-8 °C after initial use. Store protected from light), Positive Control (store at -20°C) |
| Description | RapidFinder Vegan ID Kit |
| Detection Method | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR Method | qPCR |
| Reaction Speed | Fast |
| Product Type | Vegan Identification Kit |
| For Use With (Application) | qPCR |
| No. of Reactions | 48 Reactions |
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.