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R&D Systems™ Recombinant Human Pro-EGF (aa 21-1023) Protein
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
Unit Size:
25µg
Description
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1-5 ng/mL.
Specifications
Specifications
| Accession Number | CAA28240 |
| For Use With (Application) | Bioactivity |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Gene ID (Entrez) | 1950 |
| Molecular Weight (g/mol) | M.W. (Observed): 138-145 kDa, reducing conditions; M.W. (theoretical): 112 kDa |
| Name | Pro EGF aa 21-1023 |
| Quantity | 25 μg |
| Source | Mouse myeloma cell line, NS0-derived human EGF protein Met1-Arg1023, with a C-terminal 6-His tag |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Endotoxin Concentration | <0.01 EU / 1 μg of the protein by the LAL method. |
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