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Thermo Scientific™ Phusion Blood Direct PCR Kit
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Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood.
This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning. The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions.
Features
- No need for time-consuming and expensive DNA purification steps
- Simple protocol requires minimal hands-on time
- Extremely short PCR protocol times
- Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product
Notes
- The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications
Specifications
| Concentration | 2 U/μL |
| Content And Storage | • Phusion Blood II DNA Polymerase, 40 μL • 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL • Universal Control Primer Mix, 40 μL • 50 mM EDTA, 0.5 mL • 50 mM MgCl2, 1.5 mL • DMSO, 0.5 mL Store at -20°C. |
| GC-Rich PCR Performance | High |
| PCR Method | Direct PCR |
| Polymerase | Phusion Blood II DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | Direct PCR Kit |
| Quantity | 100 reactions |
| Shipping Condition | Wet or Dry Ice |
| Hot Start | Built-In Hot Start |
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For Research Use Only. Not for use in diagnostic procedures.
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