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Applied Biosystems™ MicroSEQ™ Full Gene 16S rDNA PCR Kit

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1 Kit
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Product Code. 30000668

Brand: Applied Biosystems™ 4349155

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Description

The MicroSEQ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria.

The MicroSEQ™ Full Gene 16S rDNA PCR Kit is the PCR component of the MicroSEQ™ 16S rDNA Full Gene Identification Kit, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use the PCR kit to amplify the bacterial 16S rRNA gene, for subsequent sequencing using the MicroSEQ™ Full Gene 16S rDNA Sequencing Kit. This PCR kit does not include a protocol or a Quick Reference Card.

  • Sequence-based measurement ensures you get reproducible results in under 24 hours.
  • Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
  • Universal domain-level primers eliminate up-front optimization.
  • MicroSEQ™ ID Analysis Software ensures accurate species identification.
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
  • Requires minimal training-similar work flow as the MicroSEQ™ D2 LSU fungal kit and the MicroSeq 500 16S rDNA kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

Specifications

Content And Storage • 2X PCR Master Mix (sufficient for 20 amplifications), 3 tubes
• Positive Control DNA, 1 tube
• Negative Control DNA (water), 1 tube
• Protocol and Quick Reference Guide

After first use, store at 2°C to 8°C in a PCR clean room.
Detection Method Primer-probe
GC-Rich PCR Performance Low
Technique SBT (Sequence-Based Typing)
For Use With (Equipment) GeneAmp 9700, Veriti Thermal Cycler
Libraries Customizable, Bacterial Library (> 2000 species)
Product Line MicroSEQ
Product Type Full Gene 16S rDNA PCR Kit
Quantity 1 Kit
Shipping Condition Dry Ice
Software MicroSEQ™ ID Analysis Software
Target 16S rRNA (full gene)
Target Organism Class Bacteria
For Use With (Application) Standard PCR
Sample Type DNA (Genomic)
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For Research Use Only. Not for use in diagnostic procedures.

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