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MDM2/HDM2 Antibody (SMP14) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 19 publications
220.00€
Specifications
| Antigen | MDM2/HDM2 |
|---|---|
| Clone | SMP14 |
| Concentration | 1 mg/mL |
| Dilution | Western Blot 1:500, Immunohistochemistry 1:100-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:200-1:500 |
| Applications | Western Blot |
Description
MDM2/HDM2 Monoclonal specifically detects MDM2/HDM2 in Human, Mouse, Rat samples. It is validated for Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.Specifications
| MDM2/HDM2 | |
| 1 mg/mL | |
| Western Blot | |
| Unconjugated | |
| Mouse | |
| p53 Pathway | |
| Q00987 | |
| 4193 | |
| Synthetic peptide C-SRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2/HDM2. [UniProt# Q00987] | |
| Primary | |
| Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| SMP14 | |
| Western Blot 1:500, Immunohistochemistry 1:100-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:200-1:500 | |
| Monoclonal | |
| Purified | |
| RUO | |
| Human | |
| ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase | |
| MDM2 | |
| IgG1 κ | |
| Protein G purified | |
| The SMP14 monoclonal antibody also recognises a peptide epitope around Thr-216 of murine MDM2 (when Thr-216 is unphosphorylated) see Zhang & Prives, 2001 (PMID: 11359766) for further details. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts. |
For Research Use Only
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