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IgG2b Rat anti-Mouse, BUV395, Clone: R12-3, BD Optibuild™

Product Code. 15808079
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50 μg
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Product Code. 15808079 Supplier BD Biosciences Supplier No. 743180

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Description

Rat Monoclonal Antibody

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] /and Igh-C[b] /haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IgG2b
Classification Monoclonal
Clone R12-3
Concentration 0.2mg/mL
Conjugate Brilliant Ultraviolet 395
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias Igh-3, gamma2b, Immunoglobulin heavy constant gamma 2B
Host Species Rat
Immunogen Pooled Mouse Ig
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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