Invitrogen™ Human IL-34 (Carrier-Free) Recombinant Protein, eBioscience™

IL-34 is secreted as a dimeric protein and currently belongs to no known cytokine family. It signals via the M-CSF Receptor, also known as CSF-1R and CD115, which is shared with M-CSF. IL-34 has no amino acid sequence homology to M-CSF, but they exhibit similarities in secondary structure and share overlapping functions. IL-34 has been shown to promote myeloid survival, proliferation, and differentiation, and to play a role in osteoclast formation. IL-34 expression has been detected in various tissues and is most abundant in the spleen. Description: IL-34 is secreted as a dimeric protein and currently belongs to no known cytokine family. It signals via the M-CSF Receptor, also known as CSF-1R and CD115, which is shared with M-CSF. IL-34 has no amino acid sequence homology to M-CSF, but they exhibit similarities in secondary structure and share overlapping functions. IL-34 has been shown to promote myeloid survival, proliferation, and differentiation, and to play a role in osteoclast formation. IL-34 expression has been detected in various tissues and is most abundant in the spleen. Applications Reported: Recombinant human IL-34 is biologically active and can induce MCP-1 secretion by normal human peripheral blood cells. Applications Tested: The activity of this protein was determined by measuring MCP-1 secretion from normal human peripheral blood cells. Source: HEK293 cells (amino acids Asn21-Pro242; accession # NP_001166243); contains a C-terminal 8X His tag. Bioactivity: The activity of this protein was determined by measuring MCP-1 secretion from normal human peripheral blood cells. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >95% as determined by SDS-PAGE and HPLC. Molecular Weight: 52.5 kDa. Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water to a concentration of 0.1-1.0mg/mL. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.