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Histone H2AX, p Ser139 Antibody (3F2), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 6 publications
Brand: Novus Biologicals NB100-74435-0.025mg
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Description
Histone H2AX (p Ser139) Monoclonal antibody specifically detects Histone H2AX (p Ser139) in Human, Mouse, Bovine samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).
Specifications
| Histone H2AX (p Ser139) | |
| Monoclonal | |
| 1 mg/mL | |
| Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 1 μg 106 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500 | |
| H2A.X, H2A/X, H2AFX | |
| Mouse | |
| 15 kDa | |
| 0.025 mg | |
| Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Mitotic Regulators, Phospho Specific | |
| 3014 | |
| Human | |
| Purified |
| Western Blot | |
| 3F2 | |
| Unconjugated | |
| P16104 | |
| H2AFX | |
| This Histone H2AX [p Ser139] Antibody (3F2) was developed against a synthetic peptide sequence surrounding phosphorylated Ser139. | |
| Protein G purified | |
| RUO | |
| Primary | |
| In Western blot this antibody detects ∼17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed. Used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section. | |
| Store at -20°C. Avoid freeze/thaw cycles. | |
| IgG1 κ |
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