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IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity mini- column

Product Code. 30117605 Shop All IBA Lifesciences Products
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Quantity:
0.2 mL
Unit Size:
0.2mL
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Product Code. 30117605

Brand: IBA Lifesciences 21251005

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Description

Strep-Tactin™ 4Flow™ high capacity gravity flow mini- column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

  • Strep-Tactin™ 4Flow™ high capacity columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in gravity flow
  • Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
  • 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
  • Targeted elution: no tedious titration steps required and multiple regeneration cycles possible using 100 mM NaOH
  • Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
  • Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock

Specifications

Binding Capacity 20 mg/mL resin
Capacity (Metric) 0.2 mL
Description 4% agarose, crosslinked
Particle Size 50 to 150 μm
Form Resin Slurry
Quantity 0.2 mL
Protein Form Tetrameric protein
Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Type Gravity Flow Column
Final Product Type Affinity chromatography equipment
Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase Agarose
Format Gravity flow column
Column Type Pre-packed gravity flow column
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