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Goat F(ab')2 anti-Mouse IgG F(ab')2 Secondary Antibody (Pre-adsorbed), Novus Biologicals™
Goat Polyclonal Antibody
Brand: Novus Biologicals NBP2-61929
This item is not returnable.
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Description
IgG F(ab')2 Polyclonal antibody specifically detects IgG F(ab')2 in Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry.
Specifications
| IgG F(ab')2 | |
| Polyclonal | |
| Unconjugated | |
| Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide | |
| Mouse IgG-F(ab')2 Fragment | |
| 1 mL | |
| Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species. | |
| Store at 4C. Do not freeze. |
| Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry | |
| 0.5 mg/mL | |
| Western Blot 1:1000 - 1:20000, ELISA 1:1000 - 1:20000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry 1:200 - 1:2000 | |
| Goat | |
| Affinity Purified | |
| Secondary | |
| Mouse | |
| IgG |
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