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AcroBiosystems GMP Human SCF Protein

Product Code. 31452281
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31452281 10 mg
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Product Code. 31452281 Supplier AcroBiosystems Supplier No. GMPSCFH2510mg

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Description

Recombinant Protein

Stem Cell Factor is also known as SCF, kit-ligand, KL, steel factor, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, or SHEP7, and is a cytokine that binds to the c-Kit receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same R transcript.Soluble and transmembrane SCF is produced by fibroblasts and endothelial cells. Soluble SCF has a molecular weight of 18,5 KDa and forms a dimer. SCF plays an important role in the hematopoiesis during embryonic development. Sites where hematopoiesis takes place, such as the fetal liver and bone marrow, all express SCF. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. SCF plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF may be used along with other cytokines to culture HSCs and hematopoietic progenitors. The expansion of these cells ex-vivo (outside the body) would allow advances in bone-marrow transplantation, in which HSCs are transferred to a patient to re-establish blood formation.

Specifications

Accession Number Uniprot
Characterization SCF Structure This protein carries no tag. The protein has a calculated MW of 18.5 kDa. The protein migrates as 30 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder-GMP
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Quality Control Testing <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantity 10 mg
Source GMP Human SCF Protein (GMP-SCFH25) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 189 (Accession # ). Predicted N-terminus: Glu 26 Request for sequence
Biological Activity GMP Human SCF Protein (Cat. No. GMP-SCFH25) stimulates proliferation of Mo7e cell line. The specific activity of GMP Human SCF Protein is >5.00x10^5 IU/mg, which is calibrated against human SCF WHO International Standard (NIBSC code: 91/682) (QC tested).∣GMP Human SCF Protein (Cat. No. GMP-SCFH25), GMP Human Thrombopoietin Protein (Cat. No. GMP-THNH25), GMP Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human FGF basic Protein (Cat. No. GMP-FGCH17) and GMP Human VEGF165 Protein (Cat. No. GMP-VE5H23) could significantly promote the iPSC differentiation to HSPCSs after 14 days, highly expressed hematopoietic stem cell markers CD34 and CD45.∣GMP Human SCF Protein (Cat. No. GMP-SCFH25), Human Flt-3 Ligand Protein (Cat. No. GMP-FLLH28), GMP Human IL-3 Protein (Cat. No. GMP-L03H18) and GMP Human IL-6 Protein (Cat. No. GMP-L06H27) could support the rapid cell expansion and good cell viability of CD34+ hematopoietic stem cells (Data is the average of 3 independent experiments, error bars ± SD).∣The Cell based assay shows that GMP Human SCF Protein (Cat. No. GMP-SCFH25) is stable at 4°C for 180 days.∣The Cell based assay shows that GMP Human SCF Protein (Cat. No. GMP-SCFH25) is stable at 37°C for 24 hours.∣The Cell based assay shows that GMP Human SCF (Cat. No. GMP-SCFH25) is stable after freezing and thawing 3 times.∣The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG SCF.
Species Human
Sterility Sterile
Content And Storage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoxin Level Less than 10 EU/mg, tested by the LAL method in compliance with USP 85 and Ph. Eur. 2.6.14.
Shipping Condition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Purity or Quality Grade 0.95
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