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AcroBiosystems GMP Human FGF-7 / KGF Protein (CHO)

Product Code. 31453207
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31453207 1 mg
31452295 50 μg
31452890 500 μg
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Product Code. 31453207 Supplier AcroBiosystems Supplier No. GMPFG7H351mg

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Description

Recombinant Protein

Fibroblast growth factor (FGF) 7 (is also known as Keratinocyte growth factor (KGF)), a member of FGF family, is initially found to be secreted from mesenchymal cells to repair epithelial tissues. As a well-characterized paracrine growth factor for tissue growth and regeneration, fibroblast growth factor 7 (FGF7) is involved in a number of physiological and pathological processes, including lung disease and cancer. The stromal-derived FGFs, such as FGF7 and FGF10, control epithelial cell resident FGFR2IIIb activities, promote net tissue homeostasis, and restraint tumor cells from progression to malignancy.

Specifications

Accession Number Uniprot
Characterization FGF-7 Structure This protein carries no tag. The protein has a calculated MW of 18.9 kDa. The protein migrates as 27 kDa±3 kDa when calibrated against under reducing (R) condition (SDS-PAGE) due to glycosylation.
Format Powder-GMP
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with protectants. Contact us for customized product form or formulation.
Quality Control Testing <0.5 ng/μg of protein tested by ELISA.∣<0.02 ng/μg of protein tested by qPCR.∣Less than 10 EU/mg, tested by the rFC method in compliance with USP 86 and Ph. Eur. 2.6.32.∣Sterility testing was performed using the membrane filtration method in compliance with USP &CM and Ph. Eur. 2.6.1.∣Negative
Quantity 1 mg
Source GMP Human FGF-7 / KGF Protein (CHO) (GMP-FG7H35) is expressed from CHO cells. It contains AA Cys 32 - Thr 194 (Accession # ). Predicted N-terminus: Cys 32 Request for sequence
Biological Activity GMP Human FGF-7 / KGF Protein (CHO) (Cat. No. GMP-FG7H35) stimulates Human FGF-7 (Luc) HEK293 Reporter cells. The specific activity of GMP Human FGF-7 / KGF Protein (CHO) is >5.00 x 10^5 IU/mg, which is calibrated against human FGF-7 WHO International Standard (NIBSC code: 03/150) (QC tested).∣GMP Human FGF-7 / KGF Protein (Cat. No. GMP-FG7H35) could promote iPSCs differentiation into pancreatic progenitor cells, with high expression of PDX1 and SOX9.∣GMP Human FGF-7 / KGF Protein (Cat. No. GMP-FG7H35) and Human FGF-7 / KGF Protein, premium grade (Cat. No. FG7-H5318) could efficiently induce iPSC differentiation to PP2 (Pancreatic Progenitor), with high expression of PP2 markers (NKX6.1, PDX1, and SOX9) by flow cytometry.
Species Human
Sterility Sterile
Content And Storage -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. Upon receipt, store it immediately at -20°C or lower for long term storage.
Endotoxin Level Less than 10 EU/mg, tested by the rFC method in compliance with USP 86 and Ph. Eur. 2.6.32.
Shipping Condition This product is supplied and shipped with blue ice, please inquire the shipping cost.
Purity or Quality Grade 0.95
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