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Novus Biologicals™ Flagellin Native Protein
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Quantity:
10 μg
Dimensione della confezione:
10µg
Descripción
Binds to human & mouse TLR5. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 & matrilysin (MMP-7). Maturation of dendritic cells in in vitro & in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous & intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1ug/mouse.
Especificaciones
Especificaciones
| Accession Number | P06179 |
| Concentration | LYOPH |
| For Use With (Application) | Bioactivity, ELISA |
| Formulation | Lyophilized from PBS |
| Quantity | 10 μg |
| Storage Requirements | Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles. |
| Endotoxin Concentration | <0.01 EU/μg purified protein (LAL test; Lonza). |
| Reconstitution | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
| Research Category | Immunology, Innate Immunity |
| Purity or Quality Grade | >95% pure by SDS-PAGE |
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