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  7. Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific™ GeneJET PCR Purification Kit

Product Code. 10578360 Shop All Thermo Scientific Products
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Quantity:
250 Preps
50 Preps
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Each
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Product Code. 10578360

Brand: Thermo Scientific™ K0702

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353.60 EUR valid until 2025-12-19
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This item is not returnable. View return policy

Description

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.

Highlights

  • Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb
  • Fast: procedure takes only 5 minutes
  • Convenient: spin columns are capped and assembled with collection tubes

Applications

Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent or radioactive sequencing
  • PCR
  • In vitro transcription
  • In situ hybridization
  • Labeling
  • Cloning
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Elution Volume Max 50 μL
High-throughput Compatibility Not High-throughput Compatible (Manual)
Purification Time 5 min.
Quantity 250 Preps
Run Time 5 min.
Shipping Condition Room Temperature
Starting Material Volume ≤400 μL
Yield 25 μg (Binding capacity)
For Use With (Application) Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization
Final Product Type DNA
Isolation Technology Silica Spin Column
Sample Type PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
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For Research Use Only. Not for use in diagnostic procedures.

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