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Thermo Scientific™ Aminoallyl-dUTP Solution (50 mM)
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Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH. Aminoallyl-dUTP can be enzymatically incorporated into DNA and the resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension
Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)
Specifications
Specifications
| Concentration | 50 mM |
| Content And Storage | Aminoallyl-dUTP Solution, 50 mM (50 μL) Store at –20°C. |
| Form | Liquid |
| Polymerase | DNA Polymerase |
| Labeling Method | Direct Labeling |
| Label or Dye | Aminoallyl |
| Quantity | 50 μL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | Standard PCR, Gene Expression, Non-Radioactive Labeling |
For Research Use Only. Not for use in diagnostic procedures.
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