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  6. Fc gamma RIIA/CD32a Antibody (7.3), Novus Biologicals™

Fc gamma RIIA/CD32a Antibody (7.3), Novus Biologicals™

Mouse Monoclonal Antibody

277.00€ - 474.00€

Specifications

Antigen Fc gamma RIIA/CD32a
Clone 7.3
Concentration 0.2 mg/mL
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Applications Flow Cytometry, SDS-Page, Immunofluorescence
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18130909
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Novus Biologicals
NBP2-44721-0.02MG 		0.02 mg
277.00€
0.02mg
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18153089
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Novus Biologicals
NBP2-44721-0.1MG 		0.1 mg
474.00€
0.10mg
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Description

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

Fc gamma RIIA/CD32a Monoclonal specifically detects Fc gamma RIIA/CD32a in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence.
Specifications

Specifications

Fc gamma RIIA/CD32a
0.2 mg/mL
Flow Cytometry, SDS-Page, Immunofluorescence
Unconjugated
Mouse
Human
P12318
2212
K562 and FcgRII+L cells
Primary
Store at 4C.
40 kDa
7.3
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Monoclonal
Purified
RUO
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
CD32 antigen, CD32A, CD32MGC23887, CDw32fc-gamma-RIIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FCGR2, FCGR2A1, FcRII-a, IGFR2MGC30032, IgG Fc receptor II-a, Immunoglobulin G Fc receptor II, low affinity immunoglobulin gamma Fc region receptor II-a
FCGR2A
IgG1 κ
Protein A or G purified
This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
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