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  5. IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
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Código de producto. 15683748 Tienda IBA Lifesciences Productos
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Vector:
pASG-IBA103
pASG-IBA105
pASG-IBA123
pASG-IBA143
pASG-IBA145
pASG-IBA162
pASG-IBA164
pASG-IBA167
pASG-IBA168
pASG-IBA23
pASG-IBA3
pASG-IBA43
pASG-IBA45
pASG-IBA5
pASG-IBA62
pASG-IBA64
pASG-IBAwt1
Tamaño de la unidad:
5µg
17 product options available for selection
Product selection table with 17 available options. Use arrow keys to navigate and Enter or Space to select.
Código de producto. Vector unitSize
15683748 pASG-IBA145 5µg
15603758 pASG-IBA164 5µg
15613758 pASG-IBA167 5µg
15623748 pASG-IBA103 5µg
15623758 pASG-IBA168 5µg
15633728 pASG-IBAwt1 5µg
15633738 pASG-IBA43 5µg
15643748 pASG-IBA105 5µg
15653738 pASG-IBA45 5µg
15653748 pASG-IBA123 5µg
15663728 pASG-IBA3 5µg
15663738 pASG-IBA62 5µg
15663748 pASG-IBA143 5µg
15683728 pASG-IBA5 5µg
15683738 pASG-IBA64 5µg
15693728 pASG-IBA23 5µg
15693748 pASG-IBA162 5µg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 17 options available.
17 options
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Código de producto. 15683748 Proveedor IBA Lifesciences N.º de proveedor 54145001

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Descripción

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

  • The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
  • The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
  • The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
  • Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Especificaciones

Inducing Agent Inducible with anhydrotetracycline
Marker Gene Ampicillin
Promoter Tet
Product Type Vector
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species E.coli
Protein Tag His Tag (6x), Twin-Strep-tag
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer Forward
Quantity 5 μg
Vector pASG-IBA145
For Use With (Application) Vector for recombinant expression in E. coli
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