missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Microspheres
  5. 15352076

IBA Lifesciences CD4 Fab Streptamer™ Isolation Kit MB

Product Code. 15352076 Shop All IBA Lifesciences Products
Click to view available options
Quantity:
1 Kit
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 15352076

Brand: IBA Lifesciences 68000206

Please to purchase this item. Need a web account? Register with us today!

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on +351 21 425 33 50 for assistance
 View alternative products

This item is not returnable. View return policy

Beschreibung

CD4 Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.

  • Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 108 cells each
  • CD4 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 109 cells
  • Biotin stock solution for Streptamer™ technology; 2 x 1 mL
  • Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50 mL
  • Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 mL and 2 x 50 mL Falcon tubes.
  • Principle of Selection: Isolation of human CD4 T cells is performed by positive selection.
  • PBMCs are labeled with magnetic CD4 Fab Streptamers™.
  • Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
  • This CD4 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
  • The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
  • Biotin is removed by washing.
  • The resulting cell preparation is highly enriched with CD4+ T cells with a purity of more than 90%.
  • No depletion steps and no columns are needed.

Spezifikation

Cell Type PBMCs
Material Magnetic Microspheres Coated with Strep-Tactin
No. of Cells 1 x 109
Quantity 1 Kit
Berichtigung von Produktinhalten

Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.

Name des Produkts

Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.

Vielen Dank, dass Sie uns helfen, unsere Website zu verbessern. Ihr Feedback wurde übermittelt