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Description
Cocktail Component Clone Isotype
APC anti-Rat CD3 1F4 mIgM
PE anti-Rat CD4 OX-35 mIgG2a
The Rat T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify rat T lymphocytes by direct immunofluorescence staining with flow cytometric analysis. The OX-35 antibody reacts with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (ie, MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells (1,2). The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32-kDa alpha chain of the CD8 differentiation antigen (1,4). The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells. The IF4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes and peripheral T lymphocytes (1,3).
Specifications
| Rat T Lymphocyte Cocktail | |
| Monoclonal | |
| FITC/APC/PE | |
| Rat | |
| 50 Tests | |
| Primary | |
| IgM |
| Flow Cytometry | |
| 1F4, OX-35, OX-8 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Affinity Purified | |
| RUO | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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