Ig Goat anti-Mouse, Brilliant Violet 480, Polyclonal, BD

BD Horizon™ BV480 Goat Anti-Mouse Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Mouse Ig primary antibodies. It is reactive with the heavy and light chains of mouse primary antibodies having the IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA isotypes. Minimal background staining of human and rat cells occurs in the absence of a primary mouse antibody. In addition, the BD Horizon BV480 Goat Anti-Mouse Ig stains mouse B lymphocytes with little non-specific staining of other cells. Therefore, it is useful as a primary immunofluorescent reagent for staining mouse B cells and antibody-producing cells and as a secondary reagent for staining human and rat cells pre-stained with mouse Ig primary antibodies.

The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye has been exclusively developed by BD Biosciences as an optimal dye for imaging applications. BD Horizon BV480 has an Ex Max at 436nm and Em Max at 477 nm. The use of a mounting reagent (eg, ProLong™ Gold) is highly recommended to maximize the photostability of BV480.