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CD3 Mouse anti-Human, BD IMag-DM, Clone: HIT3A, BD

Product Code. 15826838
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Product Code. 15826838 Supplier BD Biosciences Supplier No. 552593
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Estimated Shipment: 30-07-2026
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Description

Mouse Monoclonal Antibody

BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMagnet™. CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ anti-human CD3 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3
Applications Flow Cytometry
Classification Monoclonal
Clone HIT3A
Conjugate BD IMag-DM
Description CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Purification Method Affinity Purified
Quantity 5 mL
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C
Isotype IgG2a κ
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For Research Use Only.

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