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  7. Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Product Code. 30057790 Shop All Applied Biosystems Products
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Product Code. 30057790 Supplier Applied Biosystems™ Supplier No. 4387424
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Description

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

  • Recommended for RNA pathogen amplification
  • Consistent amplification of RNA targets with high specificity and sensitivity
  • Optimized to work with your target-specific primers and probes
  • Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 25X
Content And Storage Sufficient for 500 reactions of 25 μL, contains:
• 7mL 2X RT-PCR Buffer
• 550 μL 25X RT-PCR Enzyme Mix
• 25 mL Nuclease-free Water.
Description Works on all AB platforms, but is optimized for the 7500.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method 1-step RT-qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Passive Reference Dye ROX (Pre-mixed)
Product Line AgPath-ID, Ambion
Product Type One-Step RT-PCR Reagent
Quantity 500 reactions
Reverse Transcriptase ArrayScript™
Shipping Condition Dry Ice
Sufficient For 500 Reactions
Fidelity (vs. Taq) 25 X
No. of Reactions 500 Reactions
Sample Type RNA
Volume 7 mL
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