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Thermo Scientific™ Pierce™ HeLa Protein Digest Standard
Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
| Form | Solid |
|---|---|
| Final Product Type | Peptides |
| Product Type | Protein Digest Standard |
| For Use With (Equipment) | Mass Spectrometer |
| Reagent Type | Standard |
| Starting Material | Peptides |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
| Molecular Weight (g/mol) | 217.13 |
|---|---|
| Solubility | Water |
| PEGylated | No |
Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A
Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.
| Assay | BCA Assay |
|---|---|
| Specificity | Not Target-Specific |
| Content And Storage | Store at room temperature. |
| Description | Pierce BCA Protein Assay Reagent A |
| For Use With (Equipment) | Spectrophotometer, Microplate Reader |
| Product Type | Protein Quantitation Assay |
| For Use With (Application) | Solution-based Detection, Absorbance |
| Product Line | Pierce |
| Detection Method | Colorimetric |
Thermo Scientific™ TranscriptAid T7 High Yield Transcription Kit
Get high yield in vitro transcription from DNA templates containing a T7 RNA Polymerase promoter, including linearized plasmids or PCR products.
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | EDC |
| Cleavable | No |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| PEGylated | No |
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at -20°C. |
| Description | Sulfo-SMCC |
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 436.37 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Sulfhydryl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Maleimide, Sulfo-NHS Ester |
| Spacer Arm Length | 8.3 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Pre-Diluted Set
High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
Thermo Scientific™ Mass Spectrometry Grade Proteases
Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.
| Workflow Step | Protein Digestion |
|---|---|
| For Use With (Equipment) | Mass Spectrometer |
| Grade | MS |
| Detection Method | Mass Spectrometry |
| Product Line | Pierce |
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store at -20°C protected from light and moisture. |
| Cleavable | By Thiols |
| Water Soluble | Yes |
| Form | Powder |
| Molecular Weight (g/mol) | 492.4 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Nonselective |
| Cell Permeability | No |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Spacer Arm Length | 18.2 Å |
| PEGylated | No |
Thermo Scientific™ Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride)
Optimize applications where product integrity and risk minimization are paramount with this high-quality formulation of carbodiimide crosslinker.
| Content And Storage | Upon receipt store desiccated at -20°C. |
|---|---|
| Cleavable | No |
| Water Soluble | Yes |
| Molecular Weight (g/mol) | 191.7 |
| Solubility | Water |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Heterobifunctional |
| Chemical Reactivity | Amine-Carboxyl |
| Cell Permeability | No |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | Carbodiimide |
| Spacer Arm Length | 0.0 Å |
| Product Line | Pierce |
| PEGylated | No |
Thermo Scientific™ Pierce™ Bovine Gamma Globulin Standard Ampules, 2 mg/mL
High-quality, calibrated BGG solutions (2mg/mL) to serially dilute to create protein assay standard curves to measure antibody (IgG) concentration.
Thermo Scientific™ Micro BCA™ Reagent B (MB)
Special 3-component version of our popular BCA Reagents to measure total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
| Cleavable | No |
|---|---|
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 326.26 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use |
| Product Type | Crosslinker |
| Spacer | Short (<10 Å) |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 7.7 Å |
| PEGylated | No |
Thermo Scientific™ BS(PEG)9
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.