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Thermo Scientific™ Pierce™ Horseradish Peroxidase

Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.

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Specifications

Protein Detection Systems

Content And Storage	Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Form	Powder
Label or Dye	HRP (Horseradish Peroxidase)
For Use With (Application)	Western Blotting, ELISA, Immunohistochemistry
Product Line	Pierce™

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Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

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Specifications

Western Blot Substrates and Substrate Kits

Substrate	Horseradish Peroxidase
Content And Storage	Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form	Liquid
Product Type	Substrate
Signal Duration	1 to 2 hr.
Product Line	Pierce™
Detection Method	Chemiluminescence

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

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Specifications

General Western Blot Reagents

Product Type	Transfer Buffer

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

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Western Blot Substrates and Substrate Kits

Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

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Protein Detection Systems

Thermo Scientific™ Pierce™ High Sensitivity NeutrAvidin™-HRP

Specially prepared peroxidase-conjugated NeutrAvidin protein for maximum signal amplification with high signal-to-noise ratio in a ready-to-use solution.

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Specifications

Protein Detection Systems

Specificity	Biotin
Content And Storage	Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2°C to 8°C.
Target Molecule	Biotin
Form	Liquid
Enzyme	HRP (Horseradish Peroxidase)
Label or Dye	HRP (Horseradish Peroxidase)
For Use With (Application)	Western Blotting, ELISA
Product Line	Pierce™
Detection Method	Colorimetric, Chemiluminescence, Chemifluorescence

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Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

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Western Blot Substrates and Substrate Kits

Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

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Specifications

Substrates

Substrate	Beta-Gal Substrate
Assay	Reporter Enzyme
Substrate Properties	Chemical Substrate
Content And Storage	Store at -20°C.
Format	96-well Plate
Product Type	Pierce beta-Galactosidase Assay Reagent
For Use With (Equipment)	Microplate Reader
Form	Liquid
Label or Dye	Enzyme Labeled
Target Enzyme	Beta-Galactosidase
For Use With (Application)	Absorbance
Product Line	Pierce
Detection Method	Colorimetric

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Thermo Scientific™ Pierce™ High Sensitivity Streptavidin-HRP, Pre-Diluted

Amplify signal in Western blot, ELISA or other biotin-based detection methods with this exclusive, high signal-to-noise, peroxidase-conjugated, ready-to-use streptavidin.

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Protein Detection Systems

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

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Specifications

Western Blot Substrates and Substrate Kits

Sensitivity	∼1 pg
Product Type	ECL Western Blot Detection Reagent
For Use With (Application)	Sensitive Quantitative or Confirmatory Western Blot

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

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Specifications

Western Blot Substrates and Substrate Kits

Content And Storage	2°C to 8°C
Sensitivity	∼10 pg
Product Type	ECL Western Blot Detection Reagent
For Use With (Application)	Western Blot

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Pricing and Availability
Specifications

Western Blot Substrates and Substrate Kits

Substrate	Horseradish Peroxidase
Form	Liquid
Product Type	Substrate
Signal Duration	6 to 8 hr.
Product Line	SuperSignal™
Detection Method	Chemiluminescence

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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Pricing and Availability
Specifications

Western Blot Substrates and Substrate Kits

Shipping Condition	Room Temperature
Substrate	Horseradish Peroxidase
Recommended Antibody Concentrations	2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate	HRP
Form	Liquid
Product Type	Substrate
Signal Duration	6 to 24 hr.
For Use With (Application)	Western Blot
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

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Western Blot Substrates and Substrate Kits

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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Pricing and Availability
Specifications

Western Blot Substrates and Substrate Kits

Content And Storage	Upon receipt store at 4°C. Product shipped on ice.
Sensitivity	Low picogram to mid-femtogram
Form	Liquid
Product Type	Substrate
Membrane Compatibility	Nitrocellulose, PVDF
Product Line	Pierce™
Detection Method	Chemiluminescence, Fluorescence

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