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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 22 results
1

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

EDGE
Content And Storage Upon receipt store at room temperature. Product shipped at ambient temperature.
Form Liquid
Product Line Restore
2

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
3
Promotions Available

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

EDGE
Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore
4
Promotions Available

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

5

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

EDGE
Product Type Transfer Buffer
6
Promotions Available

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

EDGE
Content And Storage Upon receipt store at room temperature.
Form Liquid
Product Line Restore
7

Cytiva ECL Select Western Blotting Detection Reagent

Highly intense signal output for detection of medium to very low protein levels. Cytiva ECL Select Western Blotting Detection Reagent is a high sensitivity reagent for chemiluminescent Western blotting detection. Includes Luminol and Peroxide solutions, sufficient for 1000cm2 membrane.

EDGE
8

Thermo Scientific™ Miser™ Antibody Extender Solution NC

Enable Western blot detection with much less primary antibody by treating nitrocellulose membrane with this reagent.

9

Thermo Scientific™ Stripping Buffer-2, 4X

Content And Storage Ambient temperatures
Form Liquid
Product Type Western Blot Stripping Buffer
Concentration 4X
10

Thermo Scientific Chemicals Nitrocellulose stripping buffer (10X)

Form Liquid
Product Type Western Blot Stripping Buffer
Concentration 10X
11
Promotions Available

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

13

Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

Product Type ECL Western Blotting Detection Reagent
Storage Requirements 18°C to 30°C
For Use With (Application) Routine and confirmatory Western blotting
14

MP Biomedicals™ 3,3',5,5'-Tetramethylbenzidine Liquid Substrate

3,3',5,5'-Tetramethylbenzide Liquid Substrate is a chromogenic substrate for Horse Radish Peroxidase (HRP). TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results.

15

Cytiva Amersham™ Reinforced Nitrocellulose Blotting Membrane

Nitrocellulose membrane, with a 0.45 μm pore size, is suitable for proteins of a wide range of molecular weights.