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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,568)
Magnetic Affinity Separation Particles (209)
Spin Columns and Gravity-flow Columns (119)
Gel Filtration Media and Columns (114)
Miscellaneous Immobilization Supports (110)
Protease Inhibitors and Phosphatase Inhibitors (101)
Cell Lysis Detergents (74)
Affinity Gels (35)
Biotin-Binding Affinity Resins and Beads (30)
Miscellaneous Immobilized Affinity Ligands (25)
In vitro Protein Expression Systems (20)
Cell Lysis Enzymes (19)

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1 – 15 of 1,487 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2
Promotions Available

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

EDGE
3
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Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

EDGE
Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor
Form Liquid
Product Type Protease Inhibitor Cocktail
Reagent Type Protease Inhibitor
Product Line Halt
4
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Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10 mL spin columns with agarose beads to perform small-scale affinity purification methods in 15- or 50-mL conical microcentrifuge tubes.

EDGE
Content And Storage Store at room temperature.
Column Type Empty Column, Polyethylene Filter
Product Line Pierce
5
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Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

EDGE
Matrix Magnetic Agarose
6
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Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads

Immunoprecipitate or affinity purify antibodies (IgG) using Protein A/G coated magnetic particles for manual or robotic magnetic separation.

EDGE
Particle Size 1 μm
Ligand Type Protein A/G
Type Magnetic Affinity Bead
Target Antibody
Content And Storage Upon receipt store at 4°C. Product shipped with an ice pack.
Protein Form Recombinant
Form Liquid
Product Line Pierce
7
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Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

EDGE
Content And Storage Upon receipt store product at 4°C. Do not freeze.
Inhibitors Phosphatase Inhibitor
Form Liquid
Product Type Phosphatase Inhibitor Cocktail
Reagent Type Phosphatase Inhibitor
Product Line Halt
8
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Thermo Scientific™ Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Easily isolate and culture highly viable functional primary cardiomyocytes

EDGE
9

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-IgG-CH1 Conjugate consists of a 14 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the CH1 domain of all four human IgG subclasses.

EDGE
Ligand Type Antibody
Type Conjugate
Target IgG-CH1
Content And Storage Store at -5°C to -30°C.
Form Liquid
Label or Dye Biotin & Other Haptens
Product Line CaptureSelect
10

Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix

Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix.

EDGE
Particle Size 65 ±10 μm
Ligand Type C-tagXL affinity ligand
Flow Rate 50 to 200 cm/hr. (Up to 2 bar)
Stationary Phase Anti-EPEA tag
Type Affinity Matrix
Matrix Agarose-based, epoxide-activated
Form Liquid Suspension
Binding Capacity 400 nmol/mL
Product Line CaptureSelect
11
Promotions Available

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

EDGE
Stationary Phase Proprietary Ligand
Type Spin Column
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Form Liquid
Column Type Affinity, Cellulose Resin
Formulation 22 mL-capacity centrifuge columns containing 1 mL resin
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce
12
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Thermo Scientific™ AEBSF Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

EDGE
13

Thermo Scientific™ CaptureSelect™ C-tagXL GoPure™ Column

CaptureSelect C-tagXL GoPure columns are ready-to-use columns designed for fast and easy purification of C-terminal tagged proteins on standard chromatography systems with high purity and yield in a single step.

EDGE
Particle Size 65 ±10 μm
Type Pre-packed Column
Content And Storage Store at 2°C to 8°C in dark/protect from light.
Matrix C-tagXL Affinity Matrix
Form Agarose Bead
Max. Operating Pressure 0.2 MPa
Bed Height 25 mm
Product Line CaptureSelect
14
Promotions Available

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Pierce Anti-DYKDDDDK Spin Columns provide a fast, convenient method for purification of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells.

EDGE
Shipping Condition Wet Ice
Stationary Phase Anti-DYKDDDDK monoclonal antibodies
Product Type Affinity Resin
15
Promotions Available

Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

EDGE