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Hot Start PCR Reagents and Kits

In hot start PCR, key reagents are prevented from reacting in the PCR mixture until optimal temperatures are met. This reduces non-specific DNA amplification that occurs naturally at lower temperatures, increasing product yield, specificity, and sensitivity.
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1

DreamTaq™ Hot Start PCR Master Mixes

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 2X
For Use With (Application) Hot-start PCR
2
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Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
3
Promotions Available

Thermo Scientific™ Phire Hot Start II PCR Master Mixes

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 2X
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Cloning,Hot-start PCR
4

DreamTaq™ Hot Start DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.

EDGE
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
5

Macherey-Nagel™ NucleoType Mouse PCR, DirectPCR kit for genotyping of mouse samples

Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood.

Activation Time Lysis 2 min; Enzyme inactivation 3 min; PCR cycling 30–90 min
Content And Storage NucleoType HotStart PCR Master Mix (2x), Lysis Buffer M, Proteinase K. Store at 4 °C or -20 °C
Format Kit
Size (Final Product) Amplicon size: up to 1000 bp
Sample Type Mouse tail clipping (1 mm), mouse ear punch (Dia. 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30)
Yield 100 - 600 ng amplicon per 10 μL PCR
Shelf Life 1 year
For Use With (Application) Genotyping
Detection Method Primer-probe
6

Macherey-Nagel™ NucleoType Seed PCR, DirectPCR kit for genotyping of seed

Time and money saving seed genotyping workflow.

Content And Storage Lysis Buffer P; Proteinase K; NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer. Store at 4°C or -20°C
Format Kit
Size (Final Product) Amplicon size up to 2000 bp
Sample Type Hard plant material like e.g., seeds from soybeen, wheat, corn, rice, as well as from moss, fern leaf, and fir needle
Reaction Speed 30 to 90 min.
Kit Type Simple sample preparation suitable for hot start PCR
For Use With (Application) Genotyping
7

Abnova™ 2 x HotTaq PCR Mix with MgCl2

HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template.

8

Thermo Scientific™ Phire™ Hot Start DNA Polymerase

Thermo Scientific™ Phire Hot Start DNA Polymerase is the older version of Phire Hot Start DNA Polymerase; for upgraded version, please see Phire Hot Start II DNA Polymerase.

9

DreamTaq™ Hot Start Green PCR Master Mix, 1000 rxns

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

10

Thermo Scientific™ Thermo-Start™ Taq DNA Polymerase PCR Kts and Master Mxes

Hot-start modified for PCR applications requiring maximum specificity and sensitivity to detect rare or complex targets and allow for room-temperature reaction setup.

11

DreamTaq™ Hot Start Green DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

12

Thermo Scientific™ Maxima Hot Start Taq DNA Polymerase

Enhance the specificity, sensitivity, and yield of DNA amplification with this hot start DNA polymerase.

13

DreamTaq™ Hot Start Green DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

14

DreamTaq™ Hot Start Green PCR Master Mix, 1000 rxns

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.

15

DreamTaq™ Hot Start Green PCR Master Mix, 200 rxns

Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.