Hot Start PCR Reagents and Kits
Hot Start PCR Reagents and Kits
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Thermo Scientific™ Phire Green Hot Start II DNA Polymerase
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | Standalone |
Product Type | Hot Start High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 50X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ Phire Green Hot Start II PCR Master Mix
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Optimize routine and high throughput PCR applications requiring speed and specifity with this novel hot start DNA polymerase.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Purity or Quality Grade | DHPLC |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 50X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ DreamTaq™ Hot Start Green PCR Master Mix
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Thermo Scientific™ Phire Hot Start II PCR Master Mix
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2 X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning |
Product Line | Phire |
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
DNA Polymerase is the product of choice for consistently robust amplification with minimal optimization steps
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Product Line | DreamTaq™ |
Invitrogen™ PCR Master Mix Starter Pack
Comprised of three different PCR Master Mixes to cover routine PCR, Hot Start PCR and High Fidelity PCR needs.
Macherey-Nagel™ NucleoType™ Mouse PCR
Kit for rapid mouse typing experiments with common samples such as tail clips, ear punch, hair, and blood.
Activation Time | Lysis 2 min; Enzyme inactivation 3 min; PCR cycling 30–90 min |
---|---|
Content And Storage | NucleoType HotStart PCR Master Mix (2x), Lysis Buffer M, Proteinase K. Store at 4 °C or -20 °C |
Format | Kit |
Size (Final Product) | Amplicon size: up to 1000 bp |
Sample Type | Mouse tail clipping (1 mm), mouse ear punch (Dia. 1 mm), mouse blood (1 μL), mouse hairs (approx. 3–30) |
Yield | 100 - 600 ng amplicon per 10 μL PCR |
Shelf Life | 1 year |
For Use With (Application) | Genotyping |
Detection Method | Primer-probe |
Content And Storage | Lysis Buffer P; Proteinase K; NucleoType HotStart PCR Master Mix (2x) containing polymerase, dNTPs, buffer, enhancer, loading dye and stabilizer. Store at 4°C or -20°C |
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Format | Kit |
Size (Final Product) | Amplicon size up to 2000 bp |
Sample Type | Hard plant material like e.g., seeds from soybeen, wheat, corn, rice, as well as from moss, fern leaf, and fir needle |
Reaction Speed | 30 to 90 min. |
Kit Type | Simple sample preparation suitable for hot start PCR |
For Use With (Application) | Genotyping |
Abnova™ 2 x HotTaq PCR Mix with MgCl2
HotTaq PCR mix is a prepared solution containing everything needed for successful PCR reaction except specific primers and DNA template.
STEMCELL Technologies STEMtaq™ Hot Start DNA Polymerase Master Mix Kit
Hot Start DNA polymerase master mix and nuclease-free water for PCR reactions
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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STEMCELL Technologies STEMtaq™ Hot Start DNA Polymerase Master Mix Kit
Hot Start DNA polymerase master mix and nuclease-free water for PCR reactions
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase PROMO
Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance.
Content And Storage | 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. Recommended storage temperature -20°C. Stable for one year from the assay date. |
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Description | Phusion™ Hot Start II High-Fidelity DNA Polymerase |
For Use With (Application) | Cloning |