ELISA Substrates and Reagents
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ PNPP Substrates
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
Thermo Scientific™ Stop Solution for TMB Substrates
Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).
Thermo Scientific™ SuperSignal™ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Thermo Scientific™ 1-Step™ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
BD TMB Substrate Reagent Set
For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.
BD OptEIA™ Human IL-15 ELISA Set
For developing ELISA for natural or recombinant human IL-15 in serum, plasma and cell culture supernatants
Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit
Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).
Thermo Scientific™ ELISA Wash Buffer (30X)
Concentrated wash buffer for efficient use of storage in the lab
Thermo Scientific™ ELISA Blocker Blocking Buffer
Increases signal-to-noise ratio and minimizes background
Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.