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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

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1 – 15 of 10,260 results
1

Gibco™ Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.

Shipping Condition Wet Ice
Protein Aliases TrypLE, R-Protease
Immunoassay Kit Format Sandwich ELISA Kit
Content And Storage • Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.
Conjugate Horseradish Peroxidase (HRP)
Interassay CV 6.20%
Intraassay CV 7.20%
Total Assay Time 1.5 hr.
Assay Sensitivity 0.032 ng/mL
Assay Range 0.78 to 50 ng/mL
Target TrypLE (R-Protease) concentration
Format Wash steps required
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye Horseradish Peroxidase (HRP)
2
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Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
3
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Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

EDGE
4
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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
5
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Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

EDGE
Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
6

Invitrogen™ Human NF-L ELISA Kit

ELISA

EDGE
Shipping Condition Wet Ice
Immunoassay Kit Format Sandwich ELISA Kit
Target Species Human,Mouse,Rat
Conjugate HRP
Interassay CV 4%
Intraassay CV 3.7%
Gene Symbol NEFL
Total Assay Time 3 hr 45 min
Assay Sensitivity 0.8 pg/mL
Storage Requirements 2-8°C
Assay Range 1.6-1200
Test Time 1 hr 20 min
Accession Number P07196, P08551, P19527
Regulatory Status RUO
Target NF-L
Kit Contents Pre-coated microwell plate, Standard, Biotinylated Detection Antibody, Streptavidin-HRP, Sample Diluent, Wash Buffer Concentrate, Assay Buffer concentrate, Stop Solution, Substrate Solution, Adhesive Plate Covers
Gene Alias 68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L68 kDa neurofilament protein; AI847934; CMT1F; CMT2E; FLJ53642; light molecular weight neurofilament protein; micro glutamic acid-rich protein; NEF3; NEFL; nefl.L; neurofilament; neurofilament 3; neurofilament L subunit; neurofilament light; neurofilament light polypeptide; neurofilament protein; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light L homeolog; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament, light polypeptide L homeolog; neurofilament-l; neurofilament-light; Nf68; NFL; N-FL; NF-L; NFM; NF-M; NLC; OTTHUMP00000196614; PPP1R110; protein phosphatase 1, regulatory subunit 110; XELAEV_18018673mg; XNF-L;
For Use With (Equipment) Absorbance Microplate Reader
Product Type ELISA
Gene ID (Entrez) 18039, 4747, 83613
Sample Type Cerebrospinal Fluid,Plasma,Serum,Supernatant
Label or Dye Biotin
7
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Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

EDGE
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
8

Thermo Scientific™ CaptureSelect™ Alexa Fluor™ 488 Anti-C-tag Conjugate

Thermo Scientific™ CaptureSelect™ Alexa Fluor™ 488 Anti-C-tag Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) with high affinity and selectivity for the 4-amino-acid 'C-tag' peptide tag E‐P‐E‐A (glutamic acid-proline-glutamic acid-alanine).

EDGE
9

R&D Systems™ Mouse Artemin DuoSet ELISA

Sufficient for at least five 96-well plates

10

Invitrogen™ ProcartaPlex™ Mouse IFN-alpha/IFN-beta Panel, 2plex

The ProcartaPlex Mouse IFN-alpha/IFN-beta Panel 2plex enables researchers to explore immune function by analyzing 2 protein targets in a single well using Luminex xMAP technology.

EDGE
11

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

12

Thermo Scientific™ CaptureSelect™ AAV9 Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ AAV9 Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAV9 Affinity Resin.

Assay ELISA
Target CaptureSelect AAV9 Ligand
Content And Storage Store in freezer (-5°C to -30°C).
Format Kit
For Use With (Equipment) Microplate Reader
13

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA

Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAVX Affinity Matrix.

Shipping Condition Dry Ice
Product Line CaptureSelect
14
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Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

15
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Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.